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Molecular Diagnosis and Diversity for Regulated Xanthomonas
Bacterial virulence factors
This DokuWiki is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology)
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Author: Ralf Koebnik
Internal reviewer:
Expert reviewer:
Class: XopAY
Family: XopAY
Prototype: XC_3176 (Xanthomonas campestris pv. campestris; strain 8004)
GenBank ID: AAY50220.1 (244 aa)
3D structure: Unknown
XopAY was originally assigned to XC_3176, a protein that was identified as a type 3 effector (see below; Yang et al., 2015). The N-terminal portion of XC_3176 is similar to XCV1197 and the C-terminal portion is similar to XCV1198. XCV1197 was shown to contain a type 3 secretion signal and was called XopAV, now classified as XopAV1 (Teper et al., 2016 ), whereas XC_3176 was classified as XopAV2.
XopAV1 ------------------------------------------------------------ XCV1197 MRREQCTKQCRRGRSCAGAACLGRWRGGIGAGCGGQRARSTRSPATLLVCLQRYVARACT XCV1198 ------------------------------------------------------------ XC_3176 ------------------------------------------------------------ XopAV2 ------------------------------------------------------------ XopAV1 -------------------------------MARIHSGEVTMRPLALTSVTRRGARREQQ XCV1197 ACALWQAPCRLFRVRQWFFGRMRHARSRWGTMARIHSGEVTMRPLALTSVTRRGARREQQ XCV1198 ------------------------------------------------------------ XC_3176 ----------------------------------------------MTSVAREAVRHDQR XopAV2 -----------------------------------------MGSFPVTSVAREAVRHDQR XopAV1 EGATVADAGPPAPANDPHANTQLAQSLPRRPVREARLRAPGTPRGVPLVNLGRTMAHLGA XCV1197 EGATVADAGPPAPANDPHANTQLAQSLPRRPTAISTMTCITRILL--------------- XCV1198 ------------------------------------------------------------ XC_3176 EAQAPREVGYSSQRGDPQANPQLKQDLSRAPVRAARLKAPRVPIGVHIANTGLQAARFAT XopAV2 EAQAPREVGYSSQRGDPQANPQLKQDLSRAPVRAARLKAPRVPIGVHIANTGLQAARFAT XopAV1 ---VGAAVWGGFAAIQAPAAAAYGYFNDDLYYKNLAVVSGYQAVGALAAFLGMGLADVLA XCV1197 ------------------------------------------------------------ XCV1198 -------------------------------------------MGALAAFLGMGLADVLA XC_3176 SAVAGPAEAAVLDAVHAPAAAAYDYAKGEVSWKKNAGVTLAPALAYAVVMGTLSAVQYLA XopAV2 SAVAGPAEAAVLDAVHAPAAAAYDYAKGEVSWKKNAGVTLAPALAYAVVMGTLSAVQYLA XopAV1 ARYVEAQSRWYEVPTKAQRASELGATEACLDQAVDMVTRLDADPPGPGMDEGERLALARD XCV1197 ------------------------------------------------------------ XCV1198 ARYVEAQSRWYEVPTKAQRASELGATEACLDHAVDMVTIFDADHPGPAMDGGEQLVLATD XC_3176 NRYLQAQSQ--TSVSKADRAKELGVTEACLDAAAAMVNKGEVAGPGGEMTEEEAAALAAD XopAV2 NRYLQAQSQ--TSVSKADRAKELGVTEACLDAAAAMVNKGEVAGPGGEMTEEEAAALAAD XopAV1 LMHLMTVERSRLPPDLWRAARGAGNDAYGLVARLLQAARARAAEEASTSSAG XCV1197 ---------------------------------------------------- XCV1198 LLHLMTVERSRLPPELWQAASGVGDDAHRLVTQLLQAARARAADEASTSSAD XC_3176 LQNLATADRSRLPPELWRAASGVGDDAYALVTQLLLAARARASAEASASSDG XopAV2 LQNLATADRSRLPPELWRAASGVGDDAYALVTQLLLAARARASAEASASSDG
The promoter and signal region of XC_3176 were fused to the HR-inducing AvrBs1 C-terminal domain lack of 58 N-terminal amino acid residues. This construct elicited an hypersensitive response on the pepper ECW-10R containing the corresponding resistance gene Bs1 in a hrcV-dependent manner (Yang et al., 2015).
A reporter GUS fusion of XC_3176 displayed GUS activities, which were significantly lower in the mutant strains ΔhrpX and ΔhrpG than that in the wild type X. campestris pv. campestris strain (Yang et al., 2015).
XC_3176 was found to be required for the full virulence of X. campestris pv. campestris strain 8004 when assayed on Chinese radish by the leaf-clipping method (Yang et al., 2015).
Unknown
Unknown
Unknown
Yes (e.g. X. campestris, X. hortorum, X. arboricola, X. fragariae)
No
Teper D, Burstein D, Salomon D, Gershovitz M, Pupko T, Sessa G (2016). Identification of novel Xanthomonas euvesicatoria type III effector proteins by a machine-learning approach. Mol. Plant Pathol. 17: 398-411. DOI: 10.1111/mpp.12288
Yang L, Su H, Yang F, Jian H, Zhou M, Jiang W, Jiang B (2015). Identification of a new type III effector XC3176 in Xanthomonas campestris pv. campestris. Wei Sheng Wu Xue Bao 55: 1264-1272. PubMed ID: 26939454. Article in Chinese.
