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Molecular Diagnosis and Diversity for Regulated Xanthomonas
Bacterial virulence factors
This DokuWiki is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology)
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Author: Ralf Koebnik
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Expert reviewer: WANTED!
Class: XopU
Family: XopU
Prototype: XOO2877 (Xanthomonas oryzae pv. oryzae; strain T7174)
GenBank ID: BAE69632.1 (985 aa)
RefSeq ID: WP_011408937.1 (985 aa)
3D structure: Unknown
XopU was discovered via genome-wide screening for effector candidates in X. oryzae pv. oryzae (Xoo) strain MAFF 311018 in accordance with three criteria: i) ORF encoding proteins homologous to effectors of P. syringae strains and X. campestris pv. vesicatoria, ii) HrpX regulons preceded by two cis elements (Plant Induced Promoter, PIP; and -10 box-like motif) or iii) proteins with an N-terminal amino acid composition similar to known effectors (more than 10% of Ser in the first 50 amino aa, no Asp or Glu residues in the first 12 aa, and an aliphatic amino acid or Pro at the third or fourth position) (Furutani et al., 2009).
TT3S-dependent translocation of XopU has been shown by a calmodulin-dependent adenylate cyclase reporter assay (Furutani et al., 2009).
Agrobacterium-mediated transient expression of both XopQ and XopX in rice cells resulted in induction of rice immune responses, which were not observed when either protein was individually expressed. A screen for Xanthomonas effectors which can suppress XopQ-XopX induced rice immune responses, led to the identification of five effectors, namely XopU, XopV, XopP, XopG and AvrBs2, that could individually suppress these immune responses. These results suggest a complex interplay of Xanthomonas T3SS effectors in suppression of both pathogen-triggered immunity and effector-triggered immunity to promote virulence on rice (Deb et al., 2020).
Deb S, Ghosh P, Patel HK, Sonti RV (2020). Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responses. Plant J., in press. DOI: 10.1111/tpj.14924
Furutani A, Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Ochiai H, Tsuge S (2009). Identification of novel type III secretion effectors in Xanthomonas oryzae pv. oryzae. Mol. Plant Microbe Interact. 22: 96-106. DOI: 10.1094/MPMI-22-1-0096
